advantages and disadvantages of slide method blood grouping

document.addEventListener(evt, handler, false); Disadvantages: they tend to sweat a lot and gain weight. The individual whose RBCs lakes D antigen (Rh. Disadvantages - Living samples will dry out and die quickly with this type of slide preparation. (a.addEventListener("DOMContentLoaded",n,!1),e.addEventListener("load",n,!1)):(e.attachEvent("onload",n),a.attachEvent("onreadystatechange",function(){"complete"===a.readyState&&t.readyCallback()})),(n=t.source||{}).concatemoji?c(n.concatemoji):n.wpemoji&&n.twemoji&&(c(n.twemoji),c(n.wpemoji)))}(window,document,window._wpemojiSettings); display: inline !important; The theory for the inheritance of the ABO blood groups was first time described by Bernstein in 1924. Share on Facebook . There is blood group specif substances in high concentration in certain conditions, as seen in the ovarian cyst, which may neutralize the anti-A and anti-B when unwashed RBCs are used. Increase productivity and communication in the company 2. Temporary slides utilize the liquid mounting method the most. For reprint rights: BSPK Syndication Service, ESTIMATION OF BLOOD GROUP BY SLIDE METHOD, Attention-Deficit/Hyperactivity Disorder (ADHD), Acute Coronary Syndrome: Causes, Symptoms, and Diagnosis, Preparation of Blood Components From Whole Blood for Transfusion, Possible causes of a bubbling feeling in the chest. One of the disadvantages of blood group O positive is that people with this type are at a higher risk for developing bone and joint problems. Its discovery goes back to 1900, when Karl Landsteiner was trying to solve the mystery that some blood transfusions are The fastest screening for Malaria is QBC method. The ABO system inheritance was suggested in 1908 and proved in 1910. Blood groups A blood group system contains antigens controlled by a single gene. } else if (window.attachEvent) { These instruments have advantages such as high throughput and high sensitivity. Start the stopwatch . Aliquam erat volutpat. If Has A Million Fans Original, The end product in precipitation reaction appears as a large, insoluble mass of visible precipitate. 4.2/5 (639 Views . RBCs may have genetic abnormalities or acquired surface abnormalities that make them polyagglutinable. (document.getElementsByTagName('head')[0]||document.getElementsByTagName('body')[0]).appendChild(wfscr); This results in four blood types: A, O, B, and AB. Among these, ABO blood grouping system is the most important system for screening, transfusion and transplantation practice today. Slide or tile method: Puncture it with needle or lancet. vertical-align: -0.1em !important; Blood Group system & Hemolytic Disease of New born, 3.inmuno hematologa.inmunologia.2011.dr hilario. It can detect an enormous variety of antibodies. Save my name, email, and website in this browser for the next time I comment. Plasmodium species because of dehemoglobinized of RBCs in thick smear. Answer (1 of 1): Advantages:You are an honest people and won't easily commitment to others . Aesthetic Portfolio Template, Reverse grouping RBC source: RBCs for the reverse grouping also from the human source from A and B groups. Airtel Broadband 499 Plan Full Details, About IOT; The Saillant System; Flow Machine. 2. Home / Uncategorized / advantages and disadvantages of slide method blood grouping. Red cells from the specimen are reacted with reagent antisera (anti-A and anti-B). Agglutination reaction is the reaction in which soluble antibody interacts with particulate (insoluble) antigen in the presence of specific electrolytes at a particular temperature and pH, resulting in clumping of particles. Purpose of Blood Collection [] Disadvantages of Capillary Blood Collection. Ust Faculty Of Ecclesiastical Studies, The blood can be taken from a vein where it is called venous blood collection or arteries where it is called arterial blood collection. As a general rule for judging the quality of a stained blood film, the laboratory must ensure that all cell types in a blood film can be identified reliably by the staining procedure. wfscr.type = 'text/javascript'; 4. If you do it under centrifugation, it will give a false-negative result. Using the edge of the second slide, slowly smear the sample creating a thin, even coating. Copyright Pillori Associates, P.A, All Rights Reserved 2014, Geotechnical Engineering Investigation and Evaluation, Creating A Positive Classroom Culture: Minute By Minute. See additional information. Blood Group: Depending on the phenotype of an individual, different individuals' blood cells express different proteins on the surface of the red blood cells in circulation for identification by the immune system of the organism. The A, B, and O antigens are present in most human body cells, including white blood cells and platelets. advantages and disadvantages of slide method blood grouping Collection is simple and relatively painless. SLIDE GROUPING ADVANTAGES: Preliminary typing tests Use during camps DISADVANTAGES: Not routine test Less sensitive Drying of reaction giving to Your antigen makeup is the key factor in determining your blood type. The SlideShare family just got bigger. Blood Groups and Red Cell Antigens. Plasma, a fluid that consists of proteins and salts, carries all blood cells through your veins. Here to separate every chromosome properly we need to drop the cell suspension from some height. Several blood grouping systems have been described in humans. It can be used in blood donation camps and in case of an emergency. })('//www.pilloriassociates.com/?wordfence_lh=1&hid=AA490C910028A55F7BFDF28BFA98B078'); One more important thing, in addition to what has been said of the value of information above, is the calculating of parasitized RBCs in a patient' Also, drying of the Advantages: It can be performed when venous blood cannot be obtained Disadvantages : Method is insensitive Method is unreliable Normal clotting time is 1 5 minutes. To ensure that serum is added to the test tube it should always be added first to the test tube or slide. Your email address will not be published. Blood group O . ESTIMATION OF BLOOD GROUP BY TUBE METHOD Test tube method is more reliable than slide test, but takes longer time and more equipment. Not all tests can be run on capillary samples. advantages and disadvantages of slide method blood grouping. Seven of them were weak antigen or reverse grouping reactions showing a negative result with the routine method. The sections are labeled as anti-A and anti-B to identify the antisera (see Figure 786.2). for only $16.05 $11/page. 2. :Chrome\/26\.0\.1410\.63 Safari\/537\.31|WordfenceTestMonBot)/.test(navigator.userAgent)){ return; } When the mother is Rh-negative and the fetus Rh-positive, the mother may be sensitized at delivery due to feto-maternal blood mixing. ; ; ; 4. If all test is negative, then the fetus is not at risk. One locus on each chromosome 9 is occupied by an A, B, or O gene. Only fertility is difficult when inhomogeneous RH factor are possessed by mother and father. Keywords: Solid-phase method, ABO blood group, RhD blood group, blood donor, conventional for only $16.05 $11/page. Thanks for the comments. The advantages of working in a group or team 1. Answer (1 of 1): Advantages:You are an honest people and won't easily commitment to others . }; I think you can follow the topic from my website. muscle contractions. The present study aims to compare the results of manual and automated techniques for blood grouping and crossmatching so as to validate the automated system effectively. Activate your 30 day free trialto unlock unlimited reading. In the case of anemic patients, there may be fewer RBCs to be tested. The presence of A, B, and H substances in the saliva is evidence of the inheritance of an A gene, B gene, and H gene. If you do a careless reading, the result will be read as a negative result. About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features Press Copyright Contact us Creators . Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. } Slide Method donors or from blood banks.Tube Method Micro-plate Method Column/Gel Centrifugation Method 1. For serum grouping, patients serum is mixed with reagent red cells of known group (available commercially or prepared in the Next: Excessive blood loss can be rectified by blood transfusion in which blood from one person donor, is transferred into the patients blood stream. Thanks for the contact. Activate your 30 day free trialto continue reading. The ABO system is the principal blood grouping system used for humans which categorises people into one of the following four groups A, B, AB, or O. In hospitals and blood group matching laboratories, there is a need to express the results of the donor or recipient blood groups. So Rh-positive means the presence of D-antigen and not related to other Rh factors. It is important for the blood cells to match while transfusing i.e. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. HISTORY ABO blood group system was discovered by Karl Landsteiner in 1900-1901. Fertility Issues. This serum is collected from individuals who have a very strong antibody titer. the disadvantages of: 1. thick blood film cannot identify the e.g. Yamamoto F. Review: ABO blood group system--ABH oligosaccharide antigens, anti-A Easy to perform. Pathology. Warmest regards Yamamoto F. Review: ABO blood group system--ABH oligosaccharide antigens, anti-A Slide method Clean the tip of a finger with spirit. can you give more explanation about anti-human globulin, Please see this link: var wfscr = document.createElement('script'); Present Perfect Just, Already, Yet Exercises Worksheets, Make sure you fill in all mandatory fields. Also, drying of the reaction mixture at the edges causes aggregation that may be mistaken for agglutination. Thin blood smear is used to differentiate the diagnosis - which parasite 1. Free access to premium services like Tuneln, Mubi and more. Easy to perform . Thanks for your comments. Land Steiner described blood groups A, B, and O. They may have the antibodies from the mother. Capillary blood collection can sometimes rupture the blood cells, producing results that are inaccurate. Advantages: -Portable. These techniques have advantages and disadvantages inherent to them. Thick = more chance to detect a parasite (more blood) but image is not clear. 2 Specimen. Rh factor . Overuse of the same area for collection can cause scarring. There are two different types of voltage control methods they are multiple voltage control method and ward Leonard control system methods or armature voltage control. In the case of an incorrect reading of the results or interpretation will give false-positive or false-negative results. The results are stable and may be read even after many hours. In comparison to the slide test, the tube test is more sensitive and reliable; therefore, it can be used conveniently for blood transfusion. In rare cases, a person may experience a more severe adverse effect, such as: low blood pressure. padding: 0 !important; Posted on . The O gene is amorphous and does not transform the H substance. You can use my website for teaching . Disadvantages It is not recommended as a routine tests because it is not reliable for weakly reactive antigens on cells and for serum typing tests with low titer anti-A/anti-B. Wedge Method/Wedge Spreader Slide/Push Smear. Plasma, a fluid that consists of proteins and salts, carries all blood cells through your veins. Advantages of Wedge Method-easier to master-commercially "precleaned" slides are generally adequate; however, occasional lot or branch . These methods are particularly useful for determination of the blood group of a fetus at risk from haemolytic disease or neonatal anemia (see Chapter 12). Popular Brands In Russia, a large clot that slides down the tube when it is inverted. In the 80% of the population who possess the secretor gene, these antigens are also soluble in the secretions and body fluids like plasma, saliva, sweat, and semen. reverse grouping cells and absorption/elution studies. This can be done on whole blood or even on clotted blood. the disadvantages of: 1. thick blood film cannot identify the e.g. Separated the serum and prepared the RBCs saline suspension. Blood group A . If there are polyagglutinable RBCs that may agglutinate by any serum protein reagents. 33 Votes) Disadvantages of Capillary Blood Collection. Blood grouping is done based on the presence of antigens on the surface of RBCs. vomiting. fainting. Tap here to review the details. May get false results in diseases like acute leukemia or non-malignant hemolytic disorder. Methods. Waggoner Road Junior High Student Portal, Thank you for this wonderful reference and guide. Unwashed RBCs in case of multiple myeloma may give false-positive results because of rouleux formation. legacy obituaries springfield, mo / fidelity foundation address boston / advantages and disadvantages of slide method blood grouping. Red cells from the specimen are reacted with reagent antisera (anti-A and anti-B). We've updated our privacy policy. In addition, since thick blood smears are not fixed, they enable parasite views outside the host cells and thus concentrated to increase chances of A1 and A2 RBCs can be used, but A1 is sufficient in most routine procedures. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. Learn faster and smarter from top experts, Download to take your learnings offline and on the go. True Wahib and % degree of parasitaemia should not be estimated / counted on buffycoat sample either. Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. The ABO genes do not code for the production of the ABO antigens. Most of the Rh-negative persons are cde/cde; this genotype is truly an Rh-negative individual. Despite the advantages it offers, working in groups almost invariably presents challenges and disadvantages in the realm of relationships. Prepare a 2-5% cell suspension in saline from unknown blood sample. The principle involves the differential passage of red cell agglutinates and free red blood cells through a dextran acrylamide gel. Using the edge of the second slide, slowly smear the sample creating a thin, even coating. 12. Problems with bleeding and infection can occur with either method. Continue with Recommended Cookies. ga('send', 'pageview'); Remove excess liquid. If From India Kindly send your contact no. The routine immunohematological tests can be performed by automated as well as manual techniques. Drugs like dextran and contrast media may cause cellular aggregation and looks like agglutination. For that pre-chilled, methanol treated slide is used so that once the cell suspension hits the slide, it is immediately fixed on the surface. These are usually low (titer) at birth for the detection until the infants are 3 to 6 months old. for (var i = 0; i < evts.length; i++) { Test tube method is more reliable than slide test, but takes longer time and more equipment. Girl Scout Login For Leaders, } Advantages Lower risk for diseases listed that people with other ABO blood groups are most prone to. for (var i = 0; i < evts.length; i++) { A and B substances are secreted in addition to H substances by groups A and B individuals, while group AB secretes A, B, and H substances. Anti-A is from blood group B, anti-B is from blood group A, and anti-A and B are from blood group O individuals. Rh system is second in importance to the ABO system. Establishing the blood group of an individual needs forward and reverse grouping. I am the author, and this is my hobby in my retired life. To create a coverslip smear preparation, a coverslip is picked up by the corner and the point is held between the thumb and forefinger in one hand. ABO antisera: See box 786.1 and Figure 786.1.if(typeof ez_ad_units != 'undefined'){ez_ad_units.push([[250,250],'bioscience_com_pk-medrectangle-3','ezslot_1',108,'0','0'])};__ez_fad_position('div-gpt-ad-bioscience_com_pk-medrectangle-3-0'); Slide test is quick and needs only simple equipment. Hello Mohamed. Mix antiserum and blood by using a separate stick or a separate corner of a slide for each section over an area about 1 inch in diameter. Hi prof. Riaz, thank you for this wonderful piece of knowledge, i will like to have a copy of this through email if possible. These are some of the chief dangers you may encounter as part of a group: It will probably take a lot of time to create, maintain, and repair the human relationships involved in a . The designation A or B refers to phenotypes, whereas AA, BO, and OO are genotyping. DETERMINATION OF BLOOD GROUP BY SLIDE METHOD 1 Principle. Need for grouping, methods of grouping, Cross-matching . It appears that you have an ad-blocker running. Blood Grouping Techniques. 806 certified writers online. We will write a custom Essay on Blood Donation Advantages and Disadvantages specifically for you. they don't like sitting down to eat - they prefer to walk around holding a plate. bally sports detroit announcers; advantages and disadvantages of slide method blood grouping High levels of proteins and fibrinogen may cause rouleux formation, which may be mistaken as agglutination. Clipping is a handy way to collect important slides you want to go back to later. Improve the individual capabilities of the members 4. Slide Bundles, etc), which included 1432 slides in total in the past 24 hours. if some one is studying drug efficacy on plasmodium the thin smear is helpful Place 1 drop of anti-A, 1 drop of anti-B, 1 drop of anti-AB,A cell, B cell, O cell and Anti-D reagent separately on a tile. Need for grouping, methods of grouping, Cross-matching. Why Is Change In Healthcare Important, Blood includes red blood cells that transport oxygen, white blood cells that combat infections, and platelets that aid in clotting when you're injured. Require less time. Put a cover slip over the sample, careful not to trap air bubbles. A clean and dry glass slide is divided into two sections with a glass marking pencil. Unlike other slide preparation methods, the slide preparation technique in karyotyping is a bit different. BIOSCIENCE. is a nonprofit organization and proceeds from Web advertising and membership fee help support our mission. Mauris id lobortis orci. ABO Blood Type Identification and Forensic Science (1900-1960) The use of blood in forensic analysis is a method for identifying individuals suspected of committing some kinds of crimes. The action of the H gene substance ultimately gives rise to ABO antigens. Lower the cover slowly, avoiding air bubbles. The ABO group system is important during blood donation or blood transfusion as mismatching of blood group can lead to clumping of red blood cells with various disorders. He postulated that each individual inherits one ABO gene from each parent; these 2 genes decide which ABO antigens are present on the RBC membrane. This gene complex R or CDe is directly passed on from generation to generation. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. (function(i,s,o,g,r,a,m){i['GoogleAnalyticsObject']=r;i[r]=i[r]||function(){ Gram-negative bacteria may give group B-like activity. donor-recipient compatibility is necessary. If you miss the hemolysis, a positive result will change into negative results. 2. width: 1em !important; Waggoner Road Junior High Student Portal, Can be used in emergency and blood camps for preliminary grouping. Blood typing can also be carried out using methods based on detection of the DNA sequence encoding blood group antigens rather than agglutination. Disadvantages It is not recommended as a routine tests because it is not reliable for weakly reactive antigens on cells and for serum typing tests with low titer anti-A/anti-B. Looks like youve clipped this slide to already. However, further improvement in adding the internal quality control may increase the test sensitivity and validity of the test results. If reagents are contaminated or expired, it will give a false-positive result. This type of discrepancy is seen in Group A2, A2B and some other subgroups of A. If you would like to change your settings or withdraw consent at any time, the link to do so is in our privacy policy accessible from our home page.. Indicators Of Water Pollution, It can be used in blood donation camps and in case of an emergency. Indeed! 5. Slides using the liquid mounting method must be stored horizontally and are difficult to make. 3. Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. In comparison to the slide test, the tube test is more sensitive and reliable; therefore, it can be used conveniently for blood transfusion. The first transfusion may not create a problem in such cases, but it will have a blood transfusion reaction in the subsequent transfusion. These effects are more . Slide test is quick and needs only simple equipment. To prepare the slide: Place a drop of fluid in the center of the slide. box-shadow: none !important; The tube method is more accurate than the slide method. For that pre-chilled, methanol treated slide is used so that once the cell suspension hits the slide, it is immediately fixed on the surface. Adagio Overview; Examples (videos) advantages and disadvantages of slide method blood groupingasbury park press classifieds. 2. With your permission, I would like to use some components of this work, to curate a in-laboratory algorithm based tool to, assist the naive medical technologist (and perhaps even junior physicians) in the investigation of ABO discrepancies. wfscr.src = url + '&r=' + Math.random(); In hospitals and blood group matching laboratories, there is a need to express the results of the donor or recipient blood groups. Advantages: You are an honest people and won't easily commitment to others . Mauris eget euismod purus, in condimentum ante. In this method, both forward (cell), as well as reverse (serum) grouping is carried out. To view the purposes they believe they have legitimate interest for, or to object to this data processing use the vendor list link below. All the tests include details about the sampling, normal values, precautions, pathophysiology, and interpretation. Typically, analysts will first use the Appearance, Behavior, and Context (ABC) Approach to Bloodstain Verification because it can be impractical to test an entire crime scene for blood. May i please use your work as reference for a class presentation? You should use this type of slide when viewing samples such as pollen, feather, or hair. This Rh system is assigned to chromosome number 1. Advantages: It can be performed when venous blood cannot be obtained Disadvantages : Method is insensitive Method is unreliable Normal clotting time is 1 - 5 minutes. True Wahib and % degree of parasitaemia should not be estimated / counted on buffycoat sample either. However, these advantages are somewhat offset by the large size and high cost of the instruments. Figure 786.2: Cell grouping by slide method. ABO blood grouping (tile method) - reverse grouping. addEvent(evts[i], logHuman); Only two antigens were known as A and B antigens; these explain four blood groups. I will be glad to reply to your queries. ABO antisera: See box 786.1 and Figure 786.1. removeEvent(evts[i], logHuman); if(/(? One of those is that O-positivity may lead to fertility issues for women, including a higher risk of . Subsurface Investigations Foundation Engineering It is less sensitive than the tube test. In this method, both forward (cell), as well as reverse (serum) grouping is carried out. convulsions. Within a few hours of collecting the blood, the microscopy test can provide valuable information. Type O plasma can only be given to type O recipients. Tasks and workload are shared 3. The blood elements (including parasites, if any) are more concentrated (app. These antibodies result from cross-reactivity and are initiated at birth upon exposure to foreign substances. Tests for the ABH secretion may help establish the true ABO group of an individual whose red blood cell antigens are poorly developed. } It has antigen-A and antigen-B and no antibodies. The person who was laking the antigens was called Rh-negative. Purpose of Blood Collection [] Method 1. A1 and A2 RBCs can be used, but A1 is sufficient in most routine procedures. There are two different types of voltage control methods they are multiple voltage control method and ward Leonard control system methods or armature voltage control. If you do over centrifugation, it will give a false-positive result. Heterogeneous grouping is a type of distribution of students among various classrooms of a certain grade within a school. Mountain Mike's Marysville, As ukasz Frckowski explains, the thick smear is used to make the diagnosis of malaria as it is a concentration technique. The teacher cannot rely in last year . Each blood group antigen is assigned a six-digit number by the ISBT. Hematopoietic and marrow stem cell transplantation in cancer is a progressive development of medicine. Please click here to view them. 3. If there is an unusual genotype antigen, A or B is expressed weakly. methods of blood detection and testing To classify blood and perform a thorough analysis, its presence must first be detected and confirmed. Advantages of Capillary Blood Collection Only a very small amount of blood is needed, which reduces the potential for problems associated with blood loss in delicate patients. 1-888-799-7710 Copyright 2012-2021 BIOSCIENCE PK, LLC. " /> of the best, was the Bollman or Hansa-Mhle unit from Germany, in which solvent percolates through oilseed flakes contained in perforated baskets moving on an endless chain. height: 1em !important; Beside that, thin smears more clearly in pictures than thick and can differentiated between different stages of parasite or species of parasites. It can be used in blood donation camps and in case of an emergency. Dante The Opera Artists; Dante Virtual Opera; Divine Comedy; About IOT. they don't like sitting down to eat - they prefer to walk around holding a plate. High degree of analytic sensitivity. Advantages of Agglutination. Dr.Janani Mathialagan, We've updated our privacy policy. Fifa 21 Realistic Career Mode Mod, Presentations can distract the audience Poorly created presentations or presentations that are too busy or have too many bells and whistles can distract the audience. Label three tubes . Posted by ; modelo del ciclo basado en el cliente; To resolve the discrepancy, several Slide method Clean the tip of a finger with spirit. ABO BLOOD GROUP SYSTEM Here to separate every chromosome properly we need to drop the cell suspension from some height. Take 3 test tubes1, 2, 3 and put a drop of anti-A (blue), anti-B (yellow) and anti-AB serum (pink) to them. Any laboratory that can perform routine hematology tests is equipped to perform a thin and thick malaria smear. He postulated that there are three blood groups. He took his blood sample and the blood sample of 6 of his colleagues in 1901. Activate your 30 day free trialto unlock unlimited reading. The basic material is a glycoprotein or glycolipids that are the backbone to which sugars are added in response to specific enzyme transferases shown by inherited parents genes. if (window.addEventListener) { this website is the best and goodies and i think he is website goodies in the world an thank you so much sir !!!!!! wfscr.async = true; Learn faster and smarter from top experts, Download to take your learnings offline and on the go. Red cells from the specimen are reacted with reagent antisera (anti-A and anti-B). I am using references and these are in consolidated form. In blood from one third of these patients, the Lee and White . This is elaborated in the following diagram. In the case of the Rh-negative mother, it should determine the fathers blood group. Here we take a look at some of the most common blood grouping techniques. Fixation is a crucial step in preparing specimens for microscopic examination. Add one drop of red cell suspension in cach test tube. EMT was better in detecting ABO-D mixed-field reaction than routine microplate method. If serum and the cells are left for a long time will give rise to rouleux formation, which may be taken as agglutination. A1 and A2 RBCs can be used, but A1 is sufficient in most routine procedures. "forward blood grouping principle""forward and reverse blood grouping slideshare""forward blood grouping tube method""advantages and disadvantages of slide m. However, it is not recommended as a routine test in blood banks since weakly reactive antigens on cells on forward grouping and low titer anti-A and anti-B on reverse grouping may be missed. Patients older than 65 will have low titer, and antibodies in the reverse grouping may be undetectable. advantages of this approach are its speed, the sharp end point, and the re- . Enjoy access to millions of ebooks, audiobooks, magazines, and more from Scribd. The presence of Rh antigen on the surface of, The individuals whose RBCs contain D antigen (Rh. The reactions are at best semi-quantitative. Now customize the name of a clipboard to store your clips. Oil Immersion Microscopy is an essential tool in examining specimens under a compound microscope. var evts = 'contextmenu dblclick drag dragend dragenter dragleave dragover dragstart drop keydown keypress keyup mousedown mousemove mouseout mouseover mouseup mousewheel scroll'.split(' ');

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